cDNA cloning
cDNA is a single-stranded segment of DNA that is complementary to the mRNA of a coding DNA segment or of a whole gene. It...
DNA Cloning
To obtain sufficient amounts of a specific DNA sequence (e.g., a gene of interest) for study, it must be selectively amplified. This is accomplished...
Automated DNA Sequencing
Large-scale DNA sequencing requires automated procedures based on fluorescence labeling of DNA and suitable detection systems. In general, a fluorescent label can be used...
Genome sequencing
Knowledge of the nucleotide sequence of a gene provides important information about its structure, function, and evolutionary relationship to other similar genes in the...
Eukaryotic gene structure
Eukaryotic genes consist of coding and noncoding segments of DNA, called exons and introns, respectively.At first glance it seems to be an unnecessary burden...
Alternative DNA Structures
Gene expression and transcription can be influenced by changes of DNA topology. However, this type of control of gene expression is relatively universal and...
DNA as Carrier of Genetic Information
Although DNA was discovered in 1869 by Friedrich Miescher as
a new, acidic, phosphorus containing substance made up of very large molecules that he...
Chemical bounds
Some Types of Chemical Bonds close to 99% of the weight of a living cell is composed of just four elements: carbon (C), hydrogen...
The Cell and Its Components
Cells are the smallest organized structural units able to maintain an individual, albeit limited, life span while carrying out a wide variety of functions....