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Restriction Analysis by Southern Blot Analysis

Dec 25, 2016

Restriction endonucleases are DNA-cleaving enzymes with defined sequences as targets. They are often simply called restriction enzymes. Since each enzyme cleaves DNA only at its specific recognition sequence, the total DNA of an individual present in nucleated cells can be cut into pieces of manageable and defined size in a reproducible way. Individual DNA fragments… read more »

Jon Brower Minnoch The fattest 6,35 tons-man in Human History

Dec 18, 2016

Jon Brower Minnoch (born September 29, 1941 in Bainbridge Island, died September 10, 1983) is an American who holds the record of the man who has lost the most weight in the world. He is also the record holder of the fattest man in human history. He lost 419 kilograms from his highest weight (estimated… read more »

NuWhite Glutathione, Intense Skin Whitening

Aug 13, 2018

NuWhite Advanced Whitening contains L-Glutathione, Collagen and Placenta. All NuWhite components are approved by U.S FDA. NuWhite is a Japan formulated supplement that has undergone thorough research and clinical studies. Its ingredients are carefully processed and formulated through Japan’s Technology to preserve purity and stability of its potency ensuring product’s effectivity. 100% HandMade Whitening Glutathione… read more »

DNA Libraries

Dec 16, 2016

A DNA library is a collection of DNA fragments that in their entirety represent the genome, that is, a particular gene being sought and all remaining DNA. It is the starting point for cloning a gene of unknown chromosomal location. To produce a library, the total DNA is digested with a restriction enzyme, and the… read more »

cDNA cloning

Nov 27, 2016

cDNA is a single-stranded segment of DNA that is complementary to the mRNA of a coding DNA segment or of a whole gene. It can be used as a probe (cDNA probe as opposed to a genomic probe) for the corresponding gene because it is complementary to coding sections (exons) of the gene. If the… read more »

DNA Cloning

Dec 26, 2016

To obtain sufficient amounts of a specific DNA sequence (e.g., a gene of interest) for study, it must be selectively amplified. This is accomplished by DNA cloning, which produces a homogeneous population of DNA fragments from a mixture of very different DNA molecules or from all the DNA of the genome. Here procedures are required… read more »

Automated DNA Sequencing

Nov 26, 2016

Large-scale DNA sequencing requires automated procedures based on fluorescence labeling of DNA and suitable detection systems. In general, a fluorescent label can be used either directly or indirectly. Direct fluorescent labels, as used in automated sequencing, are fluorophores. These are molecules that emit a distinct fluorescent color when exposed to UV light of a specific… read more »

Genome sequencing

Nov 25, 2016

Knowledge of the nucleotide sequence of a gene provides important information about its structure, function, and evolutionary relationship to other similar genes in the same or different organisms. Thus, the development in the 1970s of relatively simple methods for sequencing DNA has had a great impact on genetics. Two basic methods for DNA sequencing have… read more »

Eukaryotic gene structure

Nov 25, 2016

Eukaryotic genes consist of coding and noncoding segments of DNA, called exons and introns, respectively.At first glance it seems to be an unnecessary burden to carry DNA without obvious functions within a gene. However, it has been recognized that this has great evolutionary advantages. When parts of different genes are rearranged on new chromosomal sites… read more »

Alternative DNA Structures

Nov 23, 2016

Gene expression and transcription can be influenced by changes of DNA topology. However, this type of control of gene expression is relatively universal and non specific.Thus, it is more suitable for permanent suppression of transcription, e.g., in genes that are expressed only in certain tissues or are active only during the embroyonic period and later… read more »